Optimus Primer is managed by the Génome Québec & Montreal Heart Institute Pharmacogenomics Centre.

Optimus Primer

PCR enrichment primer design for next-generation sequencing of exonic regions. Submit a gene list and select design parameters to generate PCR primers to amplify all exons for all known isoforms of each gene.
Note that we are now using hg19 as our reference build.

Region Variable
  • Gene List
  • Feature File
  • File
  • Text Box
  • Manual
  • File Upload
Primer 3 Variables (Pass 1 of 4)
Primer 3 Variables (Pass 2 of 4)
Primer 3 Variables (Pass 3 of 4)
Primer 3 Variables (Pass 4 of 4)